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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
22/01/2014 |
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Data da última atualização: |
09/08/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
TEIXEIRA, E. W.; SANTOS, L. G. DOS; SATTLER, A.; MESSAGE, D.; ALVES, M. L. T. M. F.; MARTINS, M. F.; GRASSI-SELLA, M. L. |
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Afiliação: |
ERICA WEINSTEIN TEIXEIRA, APTA; LUBIANE GUIMARAES DOS SANTOS, UFV; ARONI SATTLER, UFV; DEJAIR MESSAGE, UFERSA; MARIA LUISA T. M. F. ALVES, APTA; MARTA FONSECA MARTINS, CNPGL; MARINA LOPES GRASSI-SELLA, USP. |
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Título: |
Nosema ceranae has been present in Brazil for more than three decades infecting africanized honey bees. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Journal of Invertebrate Pathology, v. 114, n. 3, p. 250-254, 2013. |
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DOI: |
https://doi.org/10.1016/j.jip.2013.09.002 |
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Idioma: |
Inglês |
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Conteúdo: |
Until the mid-1990s, the only microsporidium known to infect bees of the genus Apis was Nosema apis. A second species, Nosema ceranae, was first identified in 1996 from Asian honey bees; it is postulated that this parasite was transmitted from the Asian honey bee, Apis cerana, to the European honey bee, Apis mellifera. Currently, N. ceranae is found on all continents and has often been associated with honey bee colony collapse and other reports of high bee losses. Samples of Africanized drones collected in 1979, preserved in alcohol, were analyzed by light microscopy to count spores and were subjected to DNA extraction, after which duplex PCR was conducted. All molecular analyses (triplicate) indicated that the drones were infected with both N. ceranae and N. apis. PCR products were sequenced and matched to sequences reported in the GenBank (Acc. Nos. JQ639316.1 and JQ639301.1). The venation pattern of the wings of these males was compared to those of the current population living in the same area and with the pattern of drones collected in 1968 from Ribeirão Preto, SP, Brazil, from a location close to where African swarms first escaped in 1956. The morphometric results indicated that the population collected in 1979 was significantly different from the current living population, confirming its antiquity. Considering that the use of molecular tools for identifying Nosema species is relatively recent, it is possible that previous reports of infections (which used only light microscopy, without ultrastructural analysis) wrongly identified N. ceranae as N. apis. Although we can conclude that N. ceranae has been affecting Africanized honeybees in Brazil for at least 34 years, the impact of this pathogen remains unclear. MenosUntil the mid-1990s, the only microsporidium known to infect bees of the genus Apis was Nosema apis. A second species, Nosema ceranae, was first identified in 1996 from Asian honey bees; it is postulated that this parasite was transmitted from the Asian honey bee, Apis cerana, to the European honey bee, Apis mellifera. Currently, N. ceranae is found on all continents and has often been associated with honey bee colony collapse and other reports of high bee losses. Samples of Africanized drones collected in 1979, preserved in alcohol, were analyzed by light microscopy to count spores and were subjected to DNA extraction, after which duplex PCR was conducted. All molecular analyses (triplicate) indicated that the drones were infected with both N. ceranae and N. apis. PCR products were sequenced and matched to sequences reported in the GenBank (Acc. Nos. JQ639316.1 and JQ639301.1). The venation pattern of the wings of these males was compared to those of the current population living in the same area and with the pattern of drones collected in 1968 from Ribeirão Preto, SP, Brazil, from a location close to where African swarms first escaped in 1956. The morphometric results indicated that the population collected in 1979 was significantly different from the current living population, confirming its antiquity. Considering that the use of molecular tools for identifying Nosema species is relatively recent, it is possible that previous reports of infections (which used only light m... Mostrar Tudo |
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Palavras-Chave: |
Patologia; PCR. |
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Thesagro: |
Abelha Africana; Nosema Apis. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 02528naa a2200253 a 4500
001 1977041
005 2022-08-09
008 2013 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.jip.2013.09.002$2DOI
100 1 $aTEIXEIRA, E. W.
245 $aNosema ceranae has been present in Brazil for more than three decades infecting africanized honey bees.$h[electronic resource]
260 $c2013
520 $aUntil the mid-1990s, the only microsporidium known to infect bees of the genus Apis was Nosema apis. A second species, Nosema ceranae, was first identified in 1996 from Asian honey bees; it is postulated that this parasite was transmitted from the Asian honey bee, Apis cerana, to the European honey bee, Apis mellifera. Currently, N. ceranae is found on all continents and has often been associated with honey bee colony collapse and other reports of high bee losses. Samples of Africanized drones collected in 1979, preserved in alcohol, were analyzed by light microscopy to count spores and were subjected to DNA extraction, after which duplex PCR was conducted. All molecular analyses (triplicate) indicated that the drones were infected with both N. ceranae and N. apis. PCR products were sequenced and matched to sequences reported in the GenBank (Acc. Nos. JQ639316.1 and JQ639301.1). The venation pattern of the wings of these males was compared to those of the current population living in the same area and with the pattern of drones collected in 1968 from Ribeirão Preto, SP, Brazil, from a location close to where African swarms first escaped in 1956. The morphometric results indicated that the population collected in 1979 was significantly different from the current living population, confirming its antiquity. Considering that the use of molecular tools for identifying Nosema species is relatively recent, it is possible that previous reports of infections (which used only light microscopy, without ultrastructural analysis) wrongly identified N. ceranae as N. apis. Although we can conclude that N. ceranae has been affecting Africanized honeybees in Brazil for at least 34 years, the impact of this pathogen remains unclear.
650 $aAbelha Africana
650 $aNosema Apis
653 $aPatologia
653 $aPCR
700 1 $aSANTOS, L. G. DOS
700 1 $aSATTLER, A.
700 1 $aMESSAGE, D.
700 1 $aALVES, M. L. T. M. F.
700 1 $aMARTINS, M. F.
700 1 $aGRASSI-SELLA, M. L.
773 $tJournal of Invertebrate Pathology$gv. 114, n. 3, p. 250-254, 2013.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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Registro Completo
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
14/11/2012 |
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Data da última atualização: |
14/11/2012 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
NIVA, C.; NIEMEYER, J.; VIEIRA, B.; CARVALHO, A.; BROWN, G. G. |
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Afiliação: |
CINTIA CARLA NIVA, Bolsita pós-doutorado CNPQ - Embrapa Florestas; JULIA NIEMEYER, UFBA; BETANI VIEIRA, Faculdades Pequeno Príncipe; ADRIANA CARVALHO, Faculdades Pequeno Príncipe; GEORGE GARDNER BROWN, CNPF. |
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Título: |
Use of Enchytraeus crypticus in ecotoxicological tests in Brazil. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
In: INTERNATIONAL COLLOQUIUM ON SOIL ZOOLOGY, 16., 2012, Coimbra. Book of abstracts. Coimbra: University of Coimbra, 2012. |
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Páginas: |
p. 210. |
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Idioma: |
Inglês |
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Palavras-Chave: |
Brasil; Enchytraeus crypticus; Teste ecotoxicológico. |
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Thesagro: |
Solo. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/70193/1/UseOfEnchytraeus-1.pdf
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Marc: |
LEADER 00649nam a2200205 a 4500
001 1939753
005 2012-11-14
008 2012 bl uuuu u00u1 u #d
100 1 $aNIVA, C.
245 $aUse of Enchytraeus crypticus in ecotoxicological tests in Brazil.$h[electronic resource]
260 $aIn: INTERNATIONAL COLLOQUIUM ON SOIL ZOOLOGY, 16., 2012, Coimbra. Book of abstracts. Coimbra: University of Coimbra$c2012
300 $ap. 210.
650 $aSolo
653 $aBrasil
653 $aEnchytraeus crypticus
653 $aTeste ecotoxicológico
700 1 $aNIEMEYER, J.
700 1 $aVIEIRA, B.
700 1 $aCARVALHO, A.
700 1 $aBROWN, G. G.
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